Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | FBİ682 | CELL CULTURE APPLICATIONS | Compulsory | 1 | 1 | 6 |
|
Level of Course Unit |
Second Cycle |
Objectives of the Course |
To give basic information and to teach techniques about cell culture. |
Name of Lecturer(s) |
Doç. Dr. Zülal ATLI ŞEKEROĞLU Yrd. Doç. Dr. Vedat ŞEKEROĞLU |
Learning Outcomes |
1 | apply the cell culture techniques | 2 | optimize working conditions of cell/tissue laboratory | 3 | know applications of cell culture methods in genetic toxicology |
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Mode of Delivery |
Formal Education |
Prerequisites and co-requisities |
None |
Recommended Optional Programme Components |
None |
Course Contents |
Basic principles of cell and tissue culture, safety and working conditions in cell culture laboratories, cell and tissue culture methods, maintaining, preservation, and using of cultures. |
Weekly Detailed Course Contents |
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1 | Introduction of cell culture and terminology | 3 | | 2 | The devices and materials used in the cell culture laboratory, and safety | 3 | | 3 | Properties of cultured cells and the culture medium | 3 | 1 | 4 | Preparation and preserving of substances and supplies used in the cultures | 3 | | 5 | Sterilization techniques | 3 | | 6 | Types of cultures and cell lines | 2 | 1 | 7 | Cell seeding | 2 | 1 | 8 | Midterm exam | 2 | | 9 | Maintenance of cell cultures | 2 | 1 | 10 | Cell counting and passaging | 3 | | 11 | Cryopreservation and archiving of cells | 3 | | 12 | Contamination | 3 | | 13 | Thawing frozen cell suspensions and reseeding | 3 | | 14 | Cultures of specific cell lines | 3 | | 15 | Applications of cell culture methods in genetic toxicology | 4 | | 16 | Final exam | 2 | |
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Recommended or Required Reading |
Freshney R. Culture of Animal Cells: A Manual of Basic Technique. Fifth Edition Wiley-Liss, 2004.
Helgason CD, Miller C. Basic Cell Culture Protocols. Humana Press, 2005.
Topal R.Ş. Hücre Kültür Teknikleri I, II. İstanbul, 2004. |
Planned Learning Activities and Teaching Methods |
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Assessment Methods and Criteria | |
SUM | 0 | |
SUM | 0 | Yarıyıl (Yıl) İçi Etkinlikleri | 40 | Yarıyıl (Yıl) Sonu Etkinlikleri | 60 | SUM | 100 |
| Language of Instruction | Turkish | Work Placement(s) | None |
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Workload Calculation |
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Midterm Examination | 1 | 2 | 2 |
Final Examination | 1 | 2 | 2 |
Attending Lectures | 9 | 3 | 27 |
Laboratory | 6 | 2 | 12 |
Discussion | 12 | 3 | 36 |
Question-Answer | 12 | 2 | 24 |
Team/Group Work | 6 | 2 | 12 |
Observation | 8 | 1 | 8 |
Brain Storming | 10 | 2 | 20 |
Report Preparation | 4 | 2 | 8 |
Report Presentation | 8 | 1 | 8 |
Criticising Paper | 8 | 2 | 16 |
Self Study | 7 | 2 | 14 |
Individual Study for Mid term Examination | 2 | 1 | 2 |
Individual Study for Final Examination | 3 | 1 | 3 |
Reading | 8 | 2 | 16 |
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Contribution of Learning Outcomes to Programme Outcomes |
LO1 | 4 | 5 | 3 | 5 | 5 | 5 | 3 | 4 | LO2 | 4 | 4 | 3 | 5 | 5 | 5 | 4 | 5 | LO3 | 3 | 4 | 4 | 5 | 5 | 4 | 4 | 5 |
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* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Ordu University Rectorate Building ,Cumhuriyet Campus , Center / ORDU / TURKEY • Tel: +90 452 226 52 00
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