| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | FBT663 | LABORATORY TECHNIQUES IN MOLECULAR GENETICS | Compulsory | 1 | 1 | 6 |
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| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| Teaching some techniques used in molecular genetics. Working methods with biomolecules
(nucleic acids, proteins) in different organisms. Information and applications about using these molecules in molecular genetics. |
| Name of Lecturer(s) |
| Doç. Dr. Yılmaz Çiftci |
| Learning Outcomes |
| 1 | know Laboratory practices | | 2 | have skill about basic molecular genetic applications |
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| Mode of Delivery |
| Formal Education |
| Prerequisites and co-requisities |
| None |
| Recommended Optional Programme Components |
| None |
| Course Contents |
| General laboratory applications in molecular genetics. |
| Weekly Detailed Course Contents |
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| 1 | The presentation of used equipment in laboratory | | | | 2 | DNA isolation from different tissues | | | | 3 | DNA isolation from different tissues | | | | 4 | Spectrophotometrically qualitative and quantitative analysis of DNA | | | | 5 | nucleic acid concentration and quality determination with agarose gel electrophoresis | | | | 6 | PCR (polymerase chain reaction), and different applications of PCR | | | | 7 | PCR (polymerase chain reaction), and different applications of PCR | | | | 8 | Mid-term exam | | | | 9 | Control of PCR product on agarose gel | | | | 10 | Amplified DNA fragmend purification from the PCR product and gel | | | | 11 | Restriction Fragment Length Polymorphism screening technique | | | | 12 | Restriction Fragment Length Polymorphism screening technique | | | | 13 | DNA sequence analysis | | | | 14 | DNA sequence analysis | | | | 15 | Cloning in a bacterial system | | | | 16 | Final Exam | | |
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| Recommended or Required Reading |
| 1 Çiftci, Y. Lecture notes (unpublished)
Recommended Reading
2 Sambrook, J. and Russell, D. (2001) Molecular Cloning: A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, ISBN-0-87969-577-3
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| Planned Learning Activities and Teaching Methods |
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| Assessment Methods and Criteria | |
| SUM | 0 | |
| SUM | 0 | | Yarıyıl (Yıl) İçi Etkinlikleri | 40 | | Yarıyıl (Yıl) Sonu Etkinlikleri | 60 | | SUM | 100 |
| | Language of Instruction | | Turkish | | Work Placement(s) | | None |
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| Workload Calculation |
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| Midterm Examination | 1 | 2 | 2 |
| Final Examination | 1 | 2 | 2 |
| Attending Lectures | 14 | 4 | 56 |
| Laboratory | 10 | 4 | 40 |
| Experiment | 10 | 4 | 40 |
| Individual Study for Mid term Examination | 10 | 2 | 20 |
| Individual Study for Final Examination | 10 | 2 | 20 |
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| Contribution of Learning Outcomes to Programme Outcomes |
| LO1 | 3 | 4 | 4 | 4 | 3 | 2 | 4 | 3 | 4 | 4 | 3 | 3 | | LO2 | 4 | 4 | 4 | 5 | 3 | 2 | 3 | 3 | 3 | 3 | 3 | 3 |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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