Ordu University Lifelong Learning Programme

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type of Course Unit	Year of Study	Semester	Number of ECTS Credits
FBK658	BASIC PROCEDURES IN BIOCHEMISTRY LABORATORY	Compulsory	1	1	6

Level of Course Unit

Second Cycle

Objectives of the Course

The aim of the course is to provide the students a basic knowledge about basic process and procedures in biochemistry laboratory

Name of Lecturer(s)

Yrd. Doç. Dr. Melek ÇOL AYVAZ

Learning Outcomes

1	Biyokimya laboratuvarında sıklıkla kullanılan konsantrasyon birimleri ve çözelti türleri hakkında bilgi sahibi olur.
2	Biyokimya laboratuvarında sıklıkla gerçekleştirilen deneysel yöntemlerin öncelik sırası hakkında fikir yürütme kabiliyetine sahip olur.
3	Biyokimya laboratuvarında sıklıkla gerçekleştirilen deneysel yöntemler hakkında bilgi sahibi olur.
4	Öğrenci, laboratuvarda izleyeceği bir deneysel yöntem hakkında literatür araştırması yaparak takip edeceği yöntem aşamalarını belirleyebilir ve sonuçları farklı şekillerde yorumlayabilir.
5

Mode of Delivery

Formal Education

Prerequisites and co-requisities

None

Recommended Optional Programme Components

None

Course Contents

Examination and preparation of biochemical buffers, protein purification techniques, protein determination methods and determination of protein concentrations, nucleic acid isolation methods and measurement of concentrations, characterization of proteins and nucleic acids by electrophoresis, methods of enzyme activity determination and calculations

Weekly Detailed Course Contents

Week	Theoretical	Practice	Laboratory
1	Concentration Concept and Solution Preparation
2	The Importance of Biochemical Buffers Preparing Buffers
3	General Information About Proteins Protein Purification Techniques
4	Chromatographic Methods
5	Protein Detection Methods Calculation of Protein Amount
6	Isolation Methods of Nucleic Acids Calculation of Nucleic Acid Concentration
7	Electrophoretic Methods Investigation of Nucleic Acids and Proteins by Electrophoresis
8	MIDTERM EXAM
9	Enzyme Activity Determination Methods Calculation of Enzyme Activity
10	Literature Research and Discussion
11	Literature Research and Discussion
12	Literature Research and Discussion
13	Literature Research and Discussion
14	Literature Research and Discussion
15	Literature Research and Discussion
16	Final Exam

Recommended or Required Reading

Güner, S. (Editör), Temel Biyokimya Teknikleri ve Uygulamaları, 2001. Telefoncu, A., Erbil, M.K., Zihnioğlu, F., Kılınç, A. (Editörler), Biyokimya ve Moleküler Biyolojide Teknikler, 2007. Denizli, A., Küfrevioğlu, Ö.İ. (Editörler), Protein Kromatografisi ve Yeni Nesil Polimerler Telefoncu, A., Kılınç, A (Editörler), Protein Analitiği, 2009.

Planned Learning Activities and Teaching Methods

Assessment Methods and Criteria

Term (or Year) Learning Activities	Quantity	Weight
SUM		0
End Of Term (or Year) Learning Activities	Quantity	Weight
SUM		0
Yarıyıl (Yıl) İçi Etkinlikleri		40
Yarıyıl (Yıl) Sonu Etkinlikleri		60
SUM		100

Language of Instruction

Work Placement(s)

None

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Midterm Examination	1	2	2
Final Examination	1	2	2
Attending Lectures	14	3	42
Practice	4	2	8
Laboratory	6	2	12
Discussion	14	1	14
Experiment	4	2	8
Report Preparation	4	3	12
Seminar	1	15	15
Criticising Paper	4	2	8
Self Study	14	3	42
Individual Study for Mid term Examination	1	10	10
Individual Study for Final Examination	1	15	15
TOTAL WORKLOAD (hours)			190

Contribution of Learning Outcomes to Programme Outcomes

	PO 1	PO 2	PO 3	PO 4	PO 5	PO 6	PO 7	PO 8	PO 9	PO 10	PO 11	PO 12	PO 13	PO 14
LO1	3
LO2				4	4	4
LO3	2		4	5	2	3			3			5	1	3
LO4		4		4		3		2	5					5
LO5

* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High