Ordu University Lifelong Learning Programme

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type of Course Unit	Year of Study	Semester	Number of ECTS Credits
FBK624	TECHNIQUES IN PROTEIN CHEMISTRY	Compulsory	1	1	6

Level of Course Unit

Second Cycle

Objectives of the Course

To teach isolation and purification of a desired protein from different sources and methods used in determination of purity control

Name of Lecturer(s)

Yrd. Doç. Dr. Melek ÇOL AYVAZ

Learning Outcomes

1	Learn the reasons for the requirement of pure protein.
2	The idea is to learn how to choose the source to purify the appropriate protein.
3	The students learns that which purificationmethod can be used as effectively with low cost and maximum yield.
4	Have knowledge about the basic techniques used in protein purification.
5	The student learns that how to control purity of purified protein has knowledge
6	The students learns protein identification methods.

Mode of Delivery

Formal Education

Prerequisites and co-requisities

None

Recommended Optional Programme Components

None

Course Contents

Protein Isolation and Purification; Protein Detection Methods; Determination of Protein Subunit Number and Stoichiometry

Weekly Detailed Course Contents

Week	Theoretical	Practice	Laboratory
1	Structure of Amino Acids and Proteins
2	Purpose and Preliminary Planning of Protein Purification Parameters to be Considered in Protein Purification
3	Protein Purification Strategy Preliminary Separation Techniques Used in Protein Purification Cell Crushing / Homogenization / Extraction clarification
4	Preliminary Separation Techniques for Protein Purification (Continued) Extraction Chromatographic Methods
5	Preliminary Separation Techniques for Protein Purification (Continued) Electrophoretic Methods
6	Protein Characterization Functional Characterization (Effect of pH and Temperature on Enzyme Activity)
7	Purity Control of Proteins
8	MİDTERM EXAM
9	Protein Detection Methods
10	Determination of Subunit Number and Stoichiometry of Proteins
11	Working on Current Articles Related to Course Content
12	Working on Current Articles Related to Course Content
13	Working on Current Articles Related to Course Content
14	Working on Current Articles Related to Course Content
15	Working on Current Articles Related to Course Content
16	FINAL EXAM

Recommended or Required Reading

Protein Analitiği, Azmi TELEFONCU; Ali KILINÇ. Ege Üniversitesi Basımevi, Bornova-İzmir, 2009 Biyokimya ve Moleküler Biyolojide Modern Teknikler, Azmi TELEFONCU; M. Kemal ERBİL; Figen ZİHNİOĞLU; Ali KILINÇ, Ege Üniversitesi Basımevi, Bornova-İzmir, 2007

Planned Learning Activities and Teaching Methods

Assessment Methods and Criteria

Term (or Year) Learning Activities	Quantity	Weight
SUM		0
End Of Term (or Year) Learning Activities	Quantity	Weight
SUM		0
Yarıyıl (Yıl) İçi Etkinlikleri		40
Yarıyıl (Yıl) Sonu Etkinlikleri		60
SUM		100

Language of Instruction

Work Placement(s)

None

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Midterm Examination	1	2	2
Final Examination	1	2	2
Attending Lectures	14	3	42
Discussion	14	1	14
Question-Answer	14	1	14
Criticising Paper	14	2	28
Self Study	14	4	56
Individual Study for Mid term Examination	1	8	8
Individual Study for Final Examination	1	8	8
TOTAL WORKLOAD (hours)			174

Contribution of Learning Outcomes to Programme Outcomes

	PO 1	PO 2	PO 3	PO 4	PO 5	PO 6	PO 7	PO 8	PO 9	PO 10	PO 11	PO 12	PO 13	PO 14
LO1		1			2			4	3	4	1		1	2
LO2		1			2			5	3	5	3		2	2
LO3		1			3			4	3	3	2		3	3
LO4		3			5			3	3	3	2		2	5
LO5		3			5			3	2	3	3		3	1
LO6		3			2			3	1	3	4		3	1

* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High