Ordu University Lifelong Learning Programme

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type of Course Unit	Year of Study	Semester	Number of ECTS Credits
FBK620	ENZYME CHEMISTRY	Compulsory	1	2	6

Level of Course Unit

Second Cycle

Objectives of the Course

Giving detailed information about the History, Classification, Kinetics and Catalysis mechanism of Enzymes, submission examples of their use in medicine, industry and biotechnology

Name of Lecturer(s)

Yrd. Doç.Dr. Melek ÇOL AYVAZ

Learning Outcomes

1	To have detailed information about the enzymes.
2	To have knowledge about the enzyme sources and the extraction and purification of enzymes from different sources.
3	To have knowledge about the field of the use of enzymes.

Mode of Delivery

Formal Education

Prerequisites and co-requisities

None

Recommended Optional Programme Components

None

Course Contents

Sutructure and Properties of Proteins, Introductıon to Enzymes, Bioenergetics, Catalysis and Introduction to Kinetics, Enzyme Inhibition, Investigation of the active site structure, Chemical Nature of Enzyme Catalysis, Cooperativity, Allosteric Enzymes and Sigmoidal Kinetics, Investigation of Enzymes in Biological Materials, Extraction and Purification of Enzyme, Enzymes as Analytical Reagents, Methods of Enzymatic Analysis, Some of Enzymatic Analysis Applications

Weekly Detailed Course Contents

Week	Theoretical	Practice	Laboratory
1	Aminoacids Protein Sutructure and Basics of Protein Structure Properties and Biosynthesis of Proteins
2	What is the enzyme? A Brief History of Enzymes The nomenclature and classification of enzymes Specificity of Enzyme Action Monomeric and oligomeric enzymes
3	Some Bioenergetic Concepts Factors Affecting Chemical Reactions Kinetics of uncatalyzed Chemical Reaction Kinetics of Enzyme-Catalyzed Reactions
4	Kinetics of the reaction catalysed by Single Substrate Enzyme Kinetics of the Multi-Substrate Enzyme-Catalyzed Reactions
5	Enzyme Inhibition
6	Investigation of Active Region Structure
7	Chemical Nature of Enzyme Catalysis Mechanisms of Reactions catalyzed by Enzymes without a cofactor Metal-activated enzymes and Metalloenzymes Place of coenzymes in enzyme catalyzed reaction
8	Mid-term exam
9	Place of coenzymes in enzyme catalyzed reaction Cooperativity Binding of oxygen to hemoglobin
10	Allosteric Enzymes and sigmoidal kinetics Importance of sigmoidal behavior Allosteric Enzymes and Metabolic Regulation
11	Investigation of Enzymes in Biological Materials Material Selection for Investigation of Characteristics of enzymes Enzyme Assay Investigation of Distribution of enzymes in the cell Sub-Unit
12	Extraction and Purification of Enzyme Determination of Molecular Weight of Enzyme
13	Determination of Molecular Weight of Enzyme
14	Instrumental Methods of Enzymatic Analysis
15	Some of Enzymatic Analysis Applications in Medicine and Industry Biotechnological Applications of Enzymes
16	Final exam

Recommended or Required Reading

Tıp ve Fen Bilimleri Öğrencileri İçin Enzim Bilgisi, Trevor Palmer (çevirenler: Salih Cengiz, Müjgan Cengiz), Bilimsel veTeknik Yayınları Çeviri Vakfı

Planned Learning Activities and Teaching Methods

Assessment Methods and Criteria

Term (or Year) Learning Activities	Quantity	Weight
SUM		0
End Of Term (or Year) Learning Activities	Quantity	Weight
SUM		0
Yarıyıl (Yıl) İçi Etkinlikleri		40
Yarıyıl (Yıl) Sonu Etkinlikleri		60
SUM		100

Language of Instruction

Turkish

Work Placement(s)

None

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Midterm Examination	1	2	2
Final Examination	1	2	2
Attending Lectures	14	3	42
Discussion	14	0.5	7
Question-Answer	14	0.5	7
Seminar	1	30	30
Criticising Paper	3	15	45
Self Study	14	3	42
Individual Study for Homework Problems	14	2	28
Individual Study for Mid term Examination	1	10	10
Individual Study for Final Examination	1	20	20
TOTAL WORKLOAD (hours)			235

Contribution of Learning Outcomes to Programme Outcomes

	PO 1	PO 2	PO 3	PO 4	PO 5	PO 6	PO 7	PO 8	PO 9	PO 10	PO 11	PO 12	PO 13	PO 14
LO1	3											3	3	3
LO2	3											3	3	3
LO3	3											3	3	3

* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High