Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
FGM613PROTEIN PURIFICATIONCompulsory116
Level of Course Unit
Second Cycle
Objectives of the Course
The aim of the course is • to provide information about the fundamentals of protein purification • to give information about the protein purification techniques and their mechanisms • to enhance students' communication skills and critical thinking through in-class team work and presentations related to protein purification techniques.
Name of Lecturer(s)
Yrd.Doç.Dr. Bekir Gökçen MAZI
Learning Outcomes
1Learn the fundamentals of protein purification
2Understand the basic calculations in protein purification
3Gain information about the protein purification techniques
4Verilen bir konuda ulusal ve uluslararası makaleleri tarayabilme yeteneğini geliştirir
5Sözlü sunum yapabilme becerisini geliştirir
Mode of Delivery
Formal Education
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Protein purification strategy and equipment, assay to identify a target protein, extraction of protein from cells or tissue, techniques for clarifying homogenized extracts, storage of protein samples, ion exchange chromatography (IEX), hydrophobic interaction chromatography (HIC), affinity chromatography, size exclusion chromatography (SEC), electrophoresis of proteins.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Protein purification strategy and equipment
2Analysis of purity
3Clarification techniques
4Methods of disruption
5Concentration of the extract
6Storage of protein samples
7The theory of chromatography
8Mid-term exam
9Separation on the basis of chemistry (properties of proteins, solvent, and chromatography media)
10Nonaffinity absorption techniques for purifying proteins (IEX, HIC)
11Affinity procedures for purifying proteins
12Nonabsorption techniques for purifying proteins (SEC)
13Monitoring the purity of protein solutions
14Student presentations
15Student presentations
16Final exam
Recommended or Required Reading
Bonner Philip L.R, 2007. Protein Purification (First Edition), Taylor and Francis, NY, USA. Roe S. (Edt.), 2001. Protein Purification Techniques (Second Edition), Oxford University Press, Oxford, UK.
Planned Learning Activities and Teaching Methods
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
SUM0
End Of Term (or Year) Learning ActivitiesQuantityWeight
SUM0
Yarıyıl (Yıl) İçi Etkinlikleri40
Yarıyıl (Yıl) Sonu Etkinlikleri60
SUM100
Language of Instruction
Turkish
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination133
Final Examination133
Attending Lectures14342
Discussion14114
Question-Answer14114
Team/Group Work717
Observation14114
Demonstration14114
Brain Storming717
Report Preparation3927
Report Presentation313
Project Preparation188
Project Presentation111
Criticising Paper4416
Self Study14114
Individual Study for Mid term Examination166
Individual Study for Final Examination166
Reading14114
TOTAL WORKLOAD (hours)213
Contribution of Learning Outcomes to Programme Outcomes
PO
1		PO
2		PO
3		PO
4		PO
5		PO
6		PO
7		PO
8		PO
9		PO
10		PO
11		PO
12		PO
13		PO
14		PO
15		PO
16		PO
17
LO15       4        
LO255    44         
LO35       4        
LO45   55 4         
LO5   54            
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
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