| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | TAB626 | CELL AND TISSUE CULTURE IN FIELD CROPS | Compulsory | 1 | 1 | 6 |
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| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| • to give the students information about role of cell and tissue culture in plant breeding
• to provide the basic knowledge on principles of cell and tissue culture
• to teach the application of vitro culture procedures to plant hybridization
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| Name of Lecturer(s) |
| Prof. Dr. Ş.Metin KARA |
| Learning Outcomes |
| 1 | Hücre ve doku kültürünün temel ilkelerini öğrenir | | 2 | Hücre ve doku kültürünün bitki ıslahı ve bitkisel üretimdeki önemini ve pratik tekniklerini öğrenir ve bu bilgileri uygulama becerisi kazanır | | 3 | | | 4 | To have the knowledge on importance and usage of cell and tissue culture in plant breeding | | 5 | Able to explain the basic principles of cell and tissue culture | | 6 | Demonstrate the ability to work in tissue culture laboratory |
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| Mode of Delivery |
| Formal Education |
| Prerequisites and co-requisities |
| None |
| Recommended Optional Programme Components |
| None |
| Course Contents |
| • laboratory equipment and lab working principles
• preparation of culture media
• principles of micro-propagation
• isolation, inoculation and culture
• selection at tissue culture level
• to benefit of tissue culture techniques in field crops breeding
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| Weekly Detailed Course Contents |
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| 1 | The importance of tissue culture and its application areas
| | | | 2 | Laboratory equipments and lab working principles
| | | | 3 | Laboratory equipments and lab working principles
| | | | 4 | Preparation of culture media
| | | | 5 | Principles of micropropagation
| | | | 6 | Isolation, inoculation and culture of plant material
| | | | 7 | Techniques of tissue culture ; embryo culture
| | | | 8 | Midterm exam
| | | | 9 | Techniques of tissue culture; cell and callus culture
| | | | 10 | Rapid propagation of crop plants by tissue culture | | | | 11 | Production of virus free plants via tissue culture
| | | | 12 | Utilization of tissue culture in plant breeding | | | | 13 | Selection at tissue culture level | | | | 14 | Techniques of tissue culture; protoplast culture | | | | 15 | Production of secondary metabolites via tissue culture | | | | 16 | Final exam | | |
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| Recommended or Required Reading |
| Babaoğlu, M., E. Gürel, S. Özcan. 2011. Bitki Biyoteknolojisi (Doku Kültürü ve Uygulamaları), Selçuk Üniv. Vakfı yayınları, Konya
Stewart, C.N. 2012.. Bitki Biyoteknolojisi ve Genetik. Çeviri. Nobel Yayıncılık |
| Planned Learning Activities and Teaching Methods |
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| Assessment Methods and Criteria | |
| SUM | 0 | |
| SUM | 0 | | Yarıyıl (Yıl) İçi Etkinlikleri | 40 | | Yarıyıl (Yıl) Sonu Etkinlikleri | 60 | | SUM | 100 |
| | Language of Instruction | | Turkish | | Work Placement(s) | | None |
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| Workload Calculation |
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| Midterm Examination | 1 | 1 | 1 |
| Final Examination | 1 | 2 | 2 |
| Attending Lectures | 14 | 3 | 42 |
| Self Study | 14 | 7 | 98 |
| Individual Study for Mid term Examination | 1 | 10 | 10 |
| Individual Study for Final Examination | 1 | 24 | 24 |
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| Contribution of Learning Outcomes to Programme Outcomes |
| LO1 | 4 | | | | | | | LO2 | | 4 | 4 | | | | | LO3 | | | | | | | | LO4 | | | | | | | | LO5 | | | | | | | | LO6 | | | | | | |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Ordu University Rectorate Building ,Cumhuriyet Campus , Center / ORDU / TURKEY • Tel: +90 452 226 52 00
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