| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | FBİ685-14 | CYTOTOXICITY TEST SYSTEMS | Compulsory | 1 | 2 | 6 |
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| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| To explain the importance of the cell’s structural and functional integrity. Understanding the mechanism of cell death. To provide information about measuring of the cytotoxicity and evaluation of data, introducing in vitro test system used in the determination of cell viability. To present applications of cytotoxicity and cell viability in certain fields such as immune system and cancer. |
| Name of Lecturer(s) |
| Doç. Dr. Vedat ŞEKEROĞLU Doç. Dr. Zülal ATLI ŞEKEROĞLU |
| Learning Outcomes |
| 1 | Hücre canlılığının önemini ve hücre ölümü mekanizmalarını bilir | | 2 | Sitotoksisite test sistemlerini tanıyarak, bu sistemler sayesinde hücre canlılığını ölçme ve değerlendirme hakkında bilgi sahibi olur | | 3 | İmmün sistem ve kanser hücrelerinde sitotoksisite test uygulamalarını bilir | | 4 | Know the cell death mechanisms and the importance of the cell survival | | 5 | Know the cytotoxicity testing systems. Have knowledge about measuring and evaluating of cell viability by using these systems | | 6 | Know the applications of the cytotoxicity assays in the immune system and cancer cells |
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| Mode of Delivery |
| Formal Education |
| Prerequisites and co-requisities |
| None |
| Recommended Optional Programme Components |
| None |
| Course Contents |
| The importance of cell viability in terms of the cell integrity and the mechanisms triggering the cell death. The cytotoxicity assays and their applications used for determining of cell viability. Determination of immune system cytotoxicity and the cancerous cell viability and the death by using cytotoxicity assays. |
| Weekly Detailed Course Contents |
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| 1 | Importance of cell viability on account of the structure and function on cell integrity | 3 | 0 | | 2 | Examination of cell function in terms of cytotoxicity | 3 | 0 | | 3 | Termination of cell viability (necrosis, cell lysis and apoptosis) | 2 | 1 | | 4 | Cytotoxins and the mechanisms of their action on cell viability | 3 | 1 | | 5 | Variety of the cells utilized in the cytotoxicity assays in vitro | 2 | 2 | | 6 | The devices and kits used to determine the cell viability | 1 | 2 | | 7 | Measurement and evaluation of cytotoxicity | 2 | 1 | | 8 | Midterm exam | 1 | 0 | | 9 | Developmental history of the cytotoxicity testing systems | 2 | 1 | | 10 | The cytotoxicity assays, their applications and the area of their usage | 2 | 1 | | 11 | The cytotoxicity assays, their applications and the area of their usage | 3 | 1 | | 12 | Immune system cytotoxicity | 3 | 0 | | 13 | Cytotoxicity and the death of targeting cancer cells | 3 | 0 | | 14 | Cell viability studies involving the cytotoxicity assays | 3 | 1 | | 15 | Cell viability studies involving the cytotoxicity assays | 2 | 1 | | 16 | Final exam | 1 | 0 |
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| Recommended or Required Reading |
| Introduction to in vitro cytotoxicology. Mechanisms and methods. Frank A. Barile. CRC Press Inc., Florida, 1994.
Mammalian Cell Viability: Methods and Protocols. Martin J. Stoddart. Humana Press, 2011.
In vitro Toxicology. Shayne C. Gad. Taylor and Francis, New York, 2000.
Cell culture methods for in vitro toxicology. Glyn N. Stacey, Alan Doyle and Margherita Ferro. Kluwer Academic Publisher, Dordrecht, 2001.
Cell Proliferation and Apoptosis. D.Hughes and H. Mehmet. BIOS Scientific Publishers Ltd., Oxford, 2003.
Several articles published on database such as PubMed. |
| Planned Learning Activities and Teaching Methods |
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| Assessment Methods and Criteria | |
| SUM | 0 | |
| SUM | 0 | | Yarıyıl (Yıl) İçi Etkinlikleri | 40 | | Yarıyıl (Yıl) Sonu Etkinlikleri | 60 | | SUM | 100 |
| | Language of Instruction | | | Work Placement(s) | | None |
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| Workload Calculation |
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| Midterm Examination | 1 | 1 | 1 |
| Final Examination | 1 | 1 | 1 |
| Quiz | 8 | 1 | 8 |
| Attending Lectures | 10 | 3 | 30 |
| Laboratory | 12 | 1 | 12 |
| Problem Solving | 14 | 1 | 14 |
| Discussion | 14 | 1 | 14 |
| Question-Answer | 14 | 1 | 14 |
| Brain Storming | 14 | 1 | 14 |
| Project Preparation | 4 | 2.5 | 10 |
| Project Presentation | 4 | 2 | 8 |
| Criticising Paper | 10 | 2 | 20 |
| Self Study | 5 | 3 | 15 |
| Individual Study for Mid term Examination | 3 | 3 | 9 |
| Individual Study for Final Examination | 5 | 2 | 10 |
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| Contribution of Learning Outcomes to Programme Outcomes |
| LO1 | 3 | 2 | 3 | | | 2 | 1 | 1 | | LO2 | 2 | 3 | 3 | 1 | 3 | 2 | 2 | 3 | | LO3 | 3 | 4 | 4 | 2 | 4 | 4 | 3 | 3 | | LO4 | | | | | | | | | | LO5 | | | | | | | | | | LO6 | | | | | | | | |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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