| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | FBİ682 | CELL CULTURE APPLICATIONS | Compulsory | 1 | 1 | 6 |
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| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| To give basic information and to teach techniques about cell culture. |
| Name of Lecturer(s) |
| Doç. Dr. Zülal ATLI ŞEKEROĞLU Yrd. Doç. Dr. Vedat ŞEKEROĞLU |
| Learning Outcomes |
| 1 | apply the cell culture techniques | | 2 | optimize working conditions of cell/tissue laboratory | | 3 | know applications of cell culture methods in genetic toxicology |
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| Mode of Delivery |
| Formal Education |
| Prerequisites and co-requisities |
| None |
| Recommended Optional Programme Components |
| None |
| Course Contents |
| Basic principles of cell and tissue culture, safety and working conditions in cell culture laboratories, cell and tissue culture methods, maintaining, preservation, and using of cultures. |
| Weekly Detailed Course Contents |
|
| 1 | Introduction of cell culture and terminology | 3 | | | 2 | The devices and materials used in the cell culture laboratory, and safety | 3 | | | 3 | Properties of cultured cells and the culture medium | 3 | 1 | | 4 | Preparation and preserving of substances and supplies used in the cultures | 3 | | | 5 | Sterilization techniques | 3 | | | 6 | Types of cultures and cell lines | 2 | 1 | | 7 | Cell seeding | 2 | 1 | | 8 | Midterm exam | 2 | | | 9 | Maintenance of cell cultures | 2 | 1 | | 10 | Cell counting and passaging | 3 | | | 11 | Cryopreservation and archiving of cells | 3 | | | 12 | Contamination | 3 | | | 13 | Thawing frozen cell suspensions and reseeding | 3 | | | 14 | Cultures of specific cell lines | 3 | | | 15 | Applications of cell culture methods in genetic toxicology | 4 | | | 16 | Final exam | 2 | |
|
| Recommended or Required Reading |
| Freshney R. Culture of Animal Cells: A Manual of Basic Technique. Fifth Edition Wiley-Liss, 2004.
Helgason CD, Miller C. Basic Cell Culture Protocols. Humana Press, 2005.
Topal R.Ş. Hücre Kültür Teknikleri I, II. İstanbul, 2004. |
| Planned Learning Activities and Teaching Methods |
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| Assessment Methods and Criteria | |
| SUM | 0 | |
| SUM | 0 | | Yarıyıl (Yıl) İçi Etkinlikleri | 40 | | Yarıyıl (Yıl) Sonu Etkinlikleri | 60 | | SUM | 100 |
| | Language of Instruction | | Turkish | | Work Placement(s) | | None |
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| Workload Calculation |
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| Midterm Examination | 1 | 2 | 2 |
| Final Examination | 1 | 2 | 2 |
| Attending Lectures | 9 | 3 | 27 |
| Laboratory | 6 | 2 | 12 |
| Discussion | 12 | 3 | 36 |
| Question-Answer | 12 | 2 | 24 |
| Team/Group Work | 6 | 2 | 12 |
| Observation | 8 | 1 | 8 |
| Brain Storming | 10 | 2 | 20 |
| Report Preparation | 4 | 2 | 8 |
| Report Presentation | 8 | 1 | 8 |
| Criticising Paper | 8 | 2 | 16 |
| Self Study | 7 | 2 | 14 |
| Individual Study for Mid term Examination | 2 | 1 | 2 |
| Individual Study for Final Examination | 3 | 1 | 3 |
| Reading | 8 | 2 | 16 |
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| Contribution of Learning Outcomes to Programme Outcomes |
| LO1 | 4 | 5 | 3 | 5 | 5 | 5 | 3 | 4 | | LO2 | 4 | 4 | 3 | 5 | 5 | 5 | 4 | 5 | | LO3 | 3 | 4 | 4 | 5 | 5 | 4 | 4 | 5 |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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