Ordu University Lifelong Learning Programme

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type of Course Unit	Year of Study	Semester	Number of ECTS Credits
FBİ660	POLYMERASE CHAIN REACTION (PCR) TYPES	Compulsory	1	2	6

Level of Course Unit

Second Cycle

Objectives of the Course

The objective of this course different PCR techniques and to educate students about practice areas

Name of Lecturer(s)

Doç. Dr. Zeynep KOLÖREN

Learning Outcomes

1	When using this technique and PCR problems and solutions encountered different kinds of information about ways to win
2	Gain information about the differences between varieties of PCR, When you should use it, advantages and disadvantages
3	Giving seminars covering a variety of exercises that are made using PCR techniques interpreting the information they obtain and will share with them, and other friends

Mode of Delivery

Formal Education

Prerequisites and co-requisities

None

Recommended Optional Programme Components

None

Course Contents

What is PCR, Conventional PCR and application areas, Nested PCR and application areas, Real time PCR, problems encountered when applying quantitative PCR, compare with standard PCR, Taqman and multiplex real time PCR techniques and application areas, Mutation scanning and PCR

Weekly Detailed Course Contents

Week	Theoretical	Practice	Laboratory
1	What is PCR Conventional PCR and application areas
2	Nested PCR and application areas
3	Real time PCR
4	RT PCR and application areas
5	Asymmetric PCR and application areas
6	Multiple (Multiplex) PCR
7	Random Replicated Polymorphic DNA (RAPD)
8	Midterm exam
9	PCR-RFLP
10	Mutation scanning and PCR
11	Problems encountered, solutions and optimization in PCR
12	Student Seminars
13	Student Seminars
14	Student Seminars
15	Student Seminars
16	Final exam

Recommended or Required Reading

Cornel Mülhardt, 2007. Molecular Biyoloji and Genomics, Germany. Robert F. Weaver, 2008. Molecular Biyoloji. Fourth edition

Planned Learning Activities and Teaching Methods

Assessment Methods and Criteria

Term (or Year) Learning Activities	Quantity	Weight
SUM		0
End Of Term (or Year) Learning Activities	Quantity	Weight
SUM		0
Yarıyıl (Yıl) İçi Etkinlikleri		40
Yarıyıl (Yıl) Sonu Etkinlikleri		60
SUM		100

Language of Instruction

Turkish

Work Placement(s)

None

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Midterm Examination	1	2	2
Final Examination	1	2	2
Attending Lectures	14	3	42
Discussion	6	4	24
Brain Storming	6	4	24
Self Study	14	3	42
Individual Study for Mid term Examination	1	10	10
Individual Study for Final Examination	2	10	20
TOTAL WORKLOAD (hours)			166

Contribution of Learning Outcomes to Programme Outcomes

	PO 1	PO 2	PO 3	PO 4	PO 5	PO 6	PO 7	PO 8
LO1	3	2	3	3	2	3	2	2
LO2	3	2	3	3	2	4	2	2
LO3	3	2	3	3	4	3	3	3

* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High