| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | FBİ639 | CELL CULTURE AND VIROLOGY METHODS | Compulsory | 1 | 1 | 6 |
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| Level of Course Unit |
| Second Cycle |
| Objectives of the Course |
| The main objectives of this course are to provide the students basic informations about cell culture and virological methods |
| Name of Lecturer(s) |
| Yrd. Doç. Dr. Ömer ERTÜRK |
| Learning Outcomes |
| 1 | ÖÇ - 1 : Virolojik tanı hakkında ayrıntılı bilgiler bilmeli | | 2 | ÖÇ - 2 :Spesifik virolojik metotları hakkında ayrıntılı araştırmalar yapmalı | | 3 | ÖÇ - 3 :Virolojik tanıda spesifik ve güncel tekniklerin uygulamalarını bilmeli | | 4 | ÖÇ - 4 :Virolojideki güncel teknikleri kullanmalı | | 5 | | | 6 | LO - 1 : have knowledge about virological diagnosis in detail | | 7 | LO - 2 : do detail literature research on a specific virological methods | | 8 | LO - 3 : have information about applications of specific and current techniques in virological diagnosis | | 9 | LO - 4 : use current techniques in virology |
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| Mode of Delivery |
| Formal Education |
| Prerequisites and co-requisities |
| None |
| Recommended Optional Programme Components |
| None |
| Course Contents |
| Importance of virological methods, Virus isolation, microscopy, complement fixation, haemagglutination, enzyme linked immunoassay, immunofluorescense, radioimmunoassay system, neutralization, assays of viral nucleic asids. |
| Weekly Detailed Course Contents |
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| 1 | History, development and importance of tissue culture. | | | | 2 | Biology of cultured cells and the culture environment. | | | | 3 | Laboratory equipment and materials and cell culture techniques. | | | | 4 | Disaggregation of the tissue and characterization of cell lines | | | | 5 | Cloning and selection of the specific cell types | | | | 6 | Study of viruses in cell culture | | | | 7 | Virus replication and stock preparation.Virus production methods | | | | 8 | Techniques in diagnostic virology. | | | | 9 | Mid-term exam | | | | 10 | Use of radioactive isotopes in cell culture. | | | | 11 | Production of monoclonal antibodies. | | | | 12 | Presentation | | | | 13 | Presentation | | | | 14 | Presentation | | | | 15 | Current techniques in cell culture | | | | 16 | End-of-term exam | | |
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| Recommended or Required Reading |
| Freshney, R.I.2005, Culture of Animal Cells, Wiley |
| Planned Learning Activities and Teaching Methods |
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| Assessment Methods and Criteria | |
| SUM | 0 | |
| SUM | 0 | | Yarıyıl (Yıl) İçi Etkinlikleri | 40 | | Yarıyıl (Yıl) Sonu Etkinlikleri | 60 | | SUM | 100 |
| | Language of Instruction | | Turkish | | Work Placement(s) | | None |
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| Workload Calculation |
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| Laboratory | 2 | 20 | 40 |
| Discussion | 3 | 10 | 30 |
| Project Preparation | 1 | 30 | 30 |
| Writing Paper | 2 | 20 | 40 |
| Self Study | 2 | 20 | 40 |
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| Contribution of Learning Outcomes to Programme Outcomes |
| LO1 | 4 | | | | | | | | | LO2 | | 4 | | | | | | | | LO3 | | | 4 | | | | | | | LO4 | | | | 3 | | | | | | LO5 | | | | | 3 | | | | | LO6 | | | | | | | | | | LO7 | | | | | | | | | | LO8 | | | | | | | | | | LO9 | | | | | | | | |
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| * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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Ordu University Rectorate Building ,Cumhuriyet Campus , Center / ORDU / TURKEY • Tel: +90 452 226 52 00
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