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Description of Individual Course Units| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | FBT679 | CYTOGENETIC AND KARYOTYPE OF AQUATIC ORGANISMS | Compulsory | 1 | 1 | 6 |
| | Level of Course Unit | | Second Cycle | | Objectives of the Course | | Aimed that improving in vitro methods on determining chromosome structure and numbers of invertebrate, crustaceans and others live in marine and inland waters, slide preparation of their
chromosomes, staining and banding this slides and investigating them, presenting some technics about karyotyping. | | Name of Lecturer(s) | | Yrd. Doç. Dr. Serkan SAYGUN | | Learning Outcomes | | 1 | Learn the theoretical and practical knowledge about aquatic invertebrate’s, crustacean’s and other’s chromosomes | | 2 | Perform cytotaxonomically studies on aquatic organisms such as aquatic invertebrates, crustaceans for etc. | | 3 | Detect chromosome and remove karyotype as a helpful technique in being distinguished of aquatic species in systematic and taxonomic studies |
| | Mode of Delivery | | Formal Education | | Prerequisites and co-requisities | | None | | Recommended Optional Programme Components | | None | | Course Contents | | This lecture consists of information on chromosome and their fragments of aquatic organisms, cytogenetic applications such as revealing numerical and structural mutations of chromosome, cytogenetic laboratory practices contended identifying their tissues used to determining
chromosome of those organisms, slide preparations, staining- banding techniques, searching preparations on microscopy. | | Weekly Detailed Course Contents | |
| 1 | Chromosomes: Morphological structure and characteristics | | | | 2 | Introduction to cytogenetic in aquatic organisms | | | | 3 | Chromosomal mutations: Numerical and Structural chromosome mutations | | | | 4 | Introduction to chromosome analyzing techniques in Invertebrates:
prefer some tissues and their characteristics for determining chromosome and selecting | | | | 5 | In vitro methods on cytogenetic of Invertebrates: applications of mitotic
inhibitors, dissecting and deriving invertebrate tissues, fixation and hypotonic procedures | | | | 6 | In vitro methods on cytogenetic of Invertebrates: Slide preparations techniques | | | | 7 | In vitro methods on cytogenetic of Invertebrates: Staining and banding | | | | 8 | Midterm exam | | | | 9 | In vitro methods on cytogenetic of Crustaceans:
tissues characteristics for determining chromosome and selecting, mitotic inhibitors and applications | | | | 10 | In vitro methods on
cytogenetic of Crustaceans: removing tissues from an crustaceans, fixation and hypotonic procedures | | | | 11 | In vitro methods on cytogenetic of Crustaceans: Spreading and slide preparation techniques
of chromosome | | | | 12 | Conventional chromosome staining applications
on invertebrates and crustaceans, and other banding procedures | | | | 13 | General microscopy examination, determining chromosome sites, microphotography | | | | 14 | In vivo cytogenetic methods on aquatic organisms: Cell culture and direct preparations | | | | 15 | Evaluation of cytogenetic results: Image analysis software, measuring chromosome lengths,
karyotyping and making ideogram | | | | 16 | Final Exam | | |
| | Recommended or Required Reading | | 1 Pisano, E., Ozouf-Costaz, C. and F. Foresti, 2007. Fish Cytogenetic. Science Publishers, 518p.
2 Denton, T.E., 1973. Fish Chromosome Methodology, Charles C. Thomas Publisher, NewYork, 166 p.
3 Ulupınar, M ve Alaş, A., 2002. Balık Sitogenetiği ve Laboratuvar tekniği (Ders Kitabı). 371s
Recommended Reading
4 Schreck, C. B. and Moyle, P. B., 1990. Methods for Fish Biology. American Fisheries Society, Bethesta, Maryland, USA, 684p.
5 Bernard, J., 1974. Animal Cytogenetics. Vol. 4: Chordata 1: Protochordata, Cyclostomata and Pisces. Gebrüder Borntraeger, Berlin, Stuttgart, Germany, 87p.
| | Planned Learning Activities and Teaching Methods | | | Assessment Methods and Criteria | |
| SUM | 0 | |
| SUM | 0 | | Yarıyıl (Yıl) İçi Etkinlikleri | 40 | | Yarıyıl (Yıl) Sonu Etkinlikleri | 60 | | SUM | 100 |
| | Language of Instruction | | Turkish | | Work Placement(s) | | None |
| | Workload Calculation | |
| Midterm Examination | 1 | 2 | 2 | | Final Examination | 1 | 2 | 2 | | Attending Lectures | 14 | 4 | 56 | | Laboratory | 10 | 4 | 40 | | Self Study | 10 | 2 | 20 | | Individual Study for Mid term Examination | 10 | 3 | 30 | | Individual Study for Final Examination | 10 | 3 | 30 | |
| Contribution of Learning Outcomes to Programme Outcomes | | LO1 | 4 | 3 | 4 | 4 | 4 | 2 | 4 | 3 | 4 | 4 | 3 | 2 | | LO2 | 3 | 4 | 4 | 4 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 2 | | LO3 | 3 | 3 | 4 | 3 | 3 | 3 | 4 | 3 | 3 | 3 | 3 | 2 |
| | * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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