Ordu University Lifelong Learning Programme

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type of Course Unit	Year of Study	Semester	Number of ECTS Credits
FBT670	POLYMERASE CHAIN REACTION (PCR) AND APPLICATION IN FISHERIES	Compulsory	1	1	6

Level of Course Unit

Second Cycle

Objectives of the Course

It is aimed to to provide theoretical and practical knowledge to students who wants to use this technology in his lab works but do not have enough about it. In this course, the basic principles of PCR methods, application areas and application forms, along with the discussion on the different components of the reaction will be discussed. In addition, each student will be informed about the problems in the process and optimization of PCR conditions. End of the course the students are expected to have the necessary knowledge and experience to use PCR and to analyze their own data.

Name of Lecturer(s)

Doç. Dr. Yılmaz ÇİFTCİ

Learning Outcomes

1	PZR’ın temel çalışma prensiplerini anlar
2	PCR yöntemleri, genel özellikleri ve kulanım alanlarını bilir
3
4	Understand the basic principles of PCR
5	know PCR methods, general characteristics and usage
6	use PCR techniques to solve biological problems

Mode of Delivery

Formal Education

Prerequisites and co-requisities

None

Recommended Optional Programme Components

None

Course Contents

The basic principles of PCR, principles of operation, types of PCR, applications, sample preparation for PCR, reagents ant their characteristics used in PCR reactions, optimization of PCR protocols, detection and purification of PCR products, and example PCR applications

Weekly Detailed Course Contents

Week	Theoretical	Practice	Laboratory
1	Definition, history and significance of PCR
2	Basic principles and components of PCR
3	Basic principles and components of PCR
4	DNA polymerases used in PCR and their characteristics
5	Properties of Thermal cyclers and optimization of PCR reactions
6	Important properties, design and synthesis of PCR primers
7	The resulting PCR errors and error handling
8	Mid-term exam
9	PCR methods, general characteristics and usage areas
10	Multiplex PCR method and applications
11	Nested PCR method and applications
12	Reverse transcriptase PCR applications
13	Touch Down and Step Down PCR
14	Real-time PCR method and applications
15	Real-time PCR method and applications
16	End-of-term exam

Recommended or Required Reading

1. Çiftci, Y. Lecture notes (Unpublished) 2. Mullis K, Faloona F (1987) Methods in enzymology, Vol. 155 (Ed. R. Wu) Academic Press, New York and London 3. Taylor G R (1991) Polymerase Chain Reaction: basic principles and automation. Ed. McPhersen MJ, Quirke P, Taylor GR,In: PZR, A practical Approach, Oxford University Press.

Planned Learning Activities and Teaching Methods

Assessment Methods and Criteria

Term (or Year) Learning Activities	Quantity	Weight
SUM		0
End Of Term (or Year) Learning Activities	Quantity	Weight
SUM		0
Yarıyıl (Yıl) İçi Etkinlikleri		40
Yarıyıl (Yıl) Sonu Etkinlikleri		60
SUM		100

Language of Instruction

Turkish

Work Placement(s)

None

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Midterm Examination	1	2	2
Final Examination	1	2	2
Attending Lectures	14	2	28
Laboratory	14	2	28
Self Study	14	4	56
Individual Study for Homework Problems	2	20	40
Individual Study for Mid term Examination	5	2	10
Individual Study for Final Examination	5	3	15
TOTAL WORKLOAD (hours)			181

Contribution of Learning Outcomes to Programme Outcomes

	PO 1	PO 2	PO 3	PO 4	PO 5	PO 6	PO 7	PO 8	PO 9	PO 10	PO 11	PO 12
LO1	3	3		4					3		3
LO2	3	3		5			4		3	3	3
LO3
LO4
LO5
LO6

* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High