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Description of Individual Course Units| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | FBT668 | INTRODUCTION TO CHROMOSOME ANALYSIS TECHNIQUES ON THE FISHES | Compulsory | 1 | 1 | 6 |
| | Level of Course Unit | | Second Cycle | | Objectives of the Course | | In this lecture, It’ll be aimed to teach some cytogenetical and karyological basic techniques for determining structural and numerical evolutions on fish chromosome where located in the cell nucleus and made the genome, as well as basic information about the structure and function of chromosomes | | Name of Lecturer(s) | | Asst. Prof. Dr. Serkan SAYGUN | | Learning Outcomes | | 1 | Learn the theoretical and practical knowledge about fish chromosomes | | 2 | Perform cytotaxonomically studies on aquatic organisms such as fish. | | 3 | Detect chromosome and remove karyotype as a helpful technique in being distinguished of fish and other species in systematic and taxonomic studies |
| | Mode of Delivery | | Formal Education | | Prerequisites and co-requisities | | None | | Recommended Optional Programme Components | | | Course Contents | | In this lecture, It’ll be contented in which general information about the cell and chromosome, laboratory practices in studies of fish chromosomes, number and structure of fish chromosome, tissues of fishes and useful some practical treatments on determining of fish chromosomes, slide preparations, basically staining and searching chromosome sites on preparation for microscopy. | | Weekly Detailed Course Contents | |
| 1 | General information of cells and chromosomes
| | | | 2 | Introduction to laboratory applications in chromosome studies
| | | | 3 | Preparation of chemicals and solutions using in chromosome analysis techniques
| | | | 4 | In vivo methods used in fish cytogenetic studies
| | | | 5 | In vitro methods used in fish and other aquatic organisms
| | | | 6 | Tissue characteristics and their selection of fish on determination of chromosomes in
| | | | 7 | Beginning of a chromosome study: Field work preparation, selection of the sampling method of species, working equipment and solutions preparation, laboratory arrangements after sampling
| | | | 8 | Mid-Term Exam | | | | 9 | Beginning of in vivo methods in cytogenetic studies : Mitotic inhibitors and their applications
| | | | 10 | Removal tissue specimens: Hypotonic treatments and fixation procedures used respectively
| | | | 11 | The slide preparation techniques used in spreading chromosomes on slides
| | | | 12 | Traditional staining and other applications of chromosome preparations
| | | | 13 | Microscopic explorations used on identifying applications of chromosomal sites
| | | | 14 | Digital Microphotography and image analysis on chromosome counting and measuring length and their applications.
| | | | 15 | Karyotyping, arranging of the ideographs, chromosome number graphics, for etc. and assessment of findings
| | | | 16 | Final exam
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| | Recommended or Required Reading | | Ulupınar, M ve Alaş, A., 2002. Balık Sitogenetiği ve Laboratuvar tekniği (Ders Kitabı). 371s.
Denton, T.E., 1973. Fish Chromosome Methodology, Charles C. Thomas Publisher, NewYork, 166 p.
Ozouf-Costaz, C., Pisano, E., Foresti, F. And L. F. de Almeida Toledo, 2015. Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans. CRC Press, 200 p.
Sharma, A.K. and A. Sharma, 1980. Chromosome Techniques: Theory and Practice, Butterworth & Co. Ltd., 699p.
Schreck, C. B. and Moyle, P. B., 1990. Methods for Fish Biology. American Fisheries Society, Bethesta, Maryland, USA, 684p.
Bernard, J., 1974. Animal Cytogenetics. Vol. 4: Chordata 1: Protochordata, Cyclostomata and Pisces. Gebrüder Borntraeger, Berlin, Stuttgart, Germany, 87p.
| | Planned Learning Activities and Teaching Methods | | | Assessment Methods and Criteria | |
| SUM | 0 | |
| SUM | 0 | | Yarıyıl (Yıl) İçi Etkinlikleri | 40 | | Yarıyıl (Yıl) Sonu Etkinlikleri | 60 | | SUM | 100 |
| | Language of Instruction | | Turkish | | Work Placement(s) | | None |
| | Workload Calculation | |
| Midterm Examination | 1 | 2 | 2 | | Final Examination | 1 | 2 | 2 | | Attending Lectures | 14 | 4 | 56 | | Practice | 14 | 2 | 28 | | Laboratory | 10 | 2 | 20 | | Team/Group Work | 2 | 4 | 8 | | Field Work | 2 | 4 | 8 | | Criticising Paper | 5 | 4 | 20 | | Self Study | 10 | 3 | 30 | | Individual Study for Mid term Examination | 1 | 8 | 8 | | Individual Study for Final Examination | 1 | 10 | 10 | |
| Contribution of Learning Outcomes to Programme Outcomes | | LO1 | 4 | 3 | 3 | 4 | 3 | 4 | 3 | 3 | 3 | 3 | 2 | 3 | | LO2 | 3 | 3 | 3 | 3 | 4 | 3 | 3 | 3 | 3 | 3 | 2 | 3 | | LO3 | 4 | 4 | 3 | 3 | 3 | 4 | 3 | 3 | 3 | 4 | 3 | 3 |
| | * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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