Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | FBT663 | LABORATORY TECHNIQUES IN MOLECULAR GENETICS | Compulsory | 1 | 1 | 6 |
|
Level of Course Unit |
Second Cycle |
Objectives of the Course |
Teaching some techniques used in molecular genetics. Working methods with biomolecules
(nucleic acids, proteins) in different organisms. Information and applications about using these molecules in molecular genetics. |
Name of Lecturer(s) |
Doç. Dr. Yılmaz Çiftci |
Learning Outcomes |
1 | know Laboratory practices | 2 | have skill about basic molecular genetic applications |
|
Mode of Delivery |
Formal Education |
Prerequisites and co-requisities |
None |
Recommended Optional Programme Components |
None |
Course Contents |
General laboratory applications in molecular genetics. |
Weekly Detailed Course Contents |
|
1 | The presentation of used equipment in laboratory | | | 2 | DNA isolation from different tissues | | | 3 | DNA isolation from different tissues | | | 4 | Spectrophotometrically qualitative and quantitative analysis of DNA | | | 5 | nucleic acid concentration and quality determination with agarose gel electrophoresis | | | 6 | PCR (polymerase chain reaction), and different applications of PCR | | | 7 | PCR (polymerase chain reaction), and different applications of PCR | | | 8 | Mid-term exam | | | 9 | Control of PCR product on agarose gel | | | 10 | Amplified DNA fragmend purification from the PCR product and gel | | | 11 | Restriction Fragment Length Polymorphism screening technique | | | 12 | Restriction Fragment Length Polymorphism screening technique | | | 13 | DNA sequence analysis | | | 14 | DNA sequence analysis | | | 15 | Cloning in a bacterial system | | | 16 | Final Exam | | |
|
Recommended or Required Reading |
1 Çiftci, Y. Lecture notes (unpublished)
Recommended Reading
2 Sambrook, J. and Russell, D. (2001) Molecular Cloning: A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, ISBN-0-87969-577-3
|
Planned Learning Activities and Teaching Methods |
|
Assessment Methods and Criteria | |
SUM | 0 | |
SUM | 0 | Yarıyıl (Yıl) İçi Etkinlikleri | 40 | Yarıyıl (Yıl) Sonu Etkinlikleri | 60 | SUM | 100 |
| Language of Instruction | Turkish | Work Placement(s) | None |
|
Workload Calculation |
|
Midterm Examination | 1 | 2 | 2 |
Final Examination | 1 | 2 | 2 |
Attending Lectures | 14 | 4 | 56 |
Laboratory | 10 | 4 | 40 |
Experiment | 10 | 4 | 40 |
Individual Study for Mid term Examination | 10 | 2 | 20 |
Individual Study for Final Examination | 10 | 2 | 20 |
|
Contribution of Learning Outcomes to Programme Outcomes |
LO1 | 3 | 4 | 4 | 4 | 3 | 2 | 4 | 3 | 4 | 4 | 3 | 3 | LO2 | 4 | 4 | 4 | 5 | 3 | 2 | 3 | 3 | 3 | 3 | 3 | 3 |
|
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
|
|
Ordu University Rectorate Building ,Cumhuriyet Campus , Center / ORDU / TURKEY • Tel: +90 452 226 52 00
|