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Description of Individual Course Units| Course Unit Code | Course Unit Title | Type of Course Unit | Year of Study | Semester | Number of ECTS Credits | | BAH623 | IN-VITRO TECHNIQUES USED IN PLANT BREEDING | Compulsory | 1 | 1 | 6 |
| | Level of Course Unit | | Second Cycle | | Objectives of the Course | | The aim of the course is
• to provide the basic knowledge about morphological and genetic definition methods of vine species and varieties
• to give the students knowledge in the fields of plant breeding, in-vitro techniques used in plant breeding
• to teach developments in the area of plant biotechnology
| | Name of Lecturer(s) | | Yrd. Doç. Dr. Ahmet AYGÜN | | Learning Outcomes | | 1 | To have knowledge about the plant tissue and cell culture techniques like embryogenesis, organogenesis, in vitro plant regeneration, protoplast culture, somatic hybridization, haploid plant production, plant disease-free synthesis of meristem culture, micro propagation and zygotic embryo culture | | 2 | To have Learn gene transfer via Agrobacterium tumefaciens, particle gun, micro injection and protoplasts | | 3 | To have knowledge about the contribution of plant biyotechnology to breeding and cultivation of field crops | | 4 | | | 5 | |
| | Mode of Delivery | | Formal Education | | Prerequisites and co-requisities | | None | | Recommended Optional Programme Components | | None | | Course Contents | | • The conceptand context of plant breeding
• Basic definitions used in plant breeding
• In-vitro techniques used in plant breeding will be examined in detail.
| | Weekly Detailed Course Contents | |
| 1 | Basic laboratory techniques and the nutrient content of media | | | | 2 | In vitro plant regeneration via organogenesis and embryogenesis | | | | 3 | Protoplast culture and somatic hybridization | | | | 4 | Production of haploid plants | | | | 5 | The embryo rescue techniques | | | | 6 | Gene transfer to plants via Agrobacterium tumefaciens | | | | 7 | Midterm exam | | | | 8 | Gene transfer toplantsviaparticlegun | | | | 9 | Microinjectionandprotoplastusage in gen transfer | | | | 10 | Herbicide-resistant transgenic plants | | | | 11 | Insects-resistant transgenic plants | | | | 12 | Viruses-resistanttransgenicplants | | | | 13 | Viruses-resistanttransgenicplants | | | | 14 | DNA markers | | | | 15 | DNA markers | | | | 16 | Final exam | | |
| | Recommended or Required Reading | | 1 Babaoğlu, M., Gürel, E., Özcan, S. (editörler), 2002. Bitki Biyoteknolojisi. Doku Kültürleri ve Uygulamaları. S.Ü. Vakfı yayını, Konya, 374 s
Other References
1 Özcan, S., Gürel, E., Babaoğlu, M. (2001) Bitki Biyoteknolojisi II: Genetik Mühendisliği ve Uygulamaları. S.Ü. Vakfı Yayınları, Konya.
2 Bains, W. , 1998. Biotechnology From A to Z. Second Edition. Oxford Univ. Pres, 411 p
3 Bull, A.,Holt, G., Lilly, M.D., (Çeviren: M. Sara), 1987. Biyoteknoloji. Uluslar arası Eğilimler ve Görüşler. İ.Ü. Fen Fak. Yayın No:197, 96 s.
4 Dixon, R.A.(Editor), 1998. Plant Cell Culture. A Practical Approach.IRL Pres, Oxford, Washington D.C., 236 p
| | Planned Learning Activities and Teaching Methods | | | Assessment Methods and Criteria | |
| SUM | 0 | |
| SUM | 0 | | Yarıyıl (Yıl) İçi Etkinlikleri | 40 | | Yarıyıl (Yıl) Sonu Etkinlikleri | 60 | | SUM | 100 |
| | Language of Instruction | | | Work Placement(s) | | None |
| | Workload Calculation | |
| Midterm Examination | 1 | 2 | 2 | | Final Examination | 1 | 2 | 2 | | Quiz | 1 | 2 | 2 | | Attending Lectures | 14 | 4 | 56 | | Practice | 5 | 4 | 20 | | Field Trip | 3 | 6 | 18 | | Individual Study for Mid term Examination | 15 | 3 | 45 | | Individual Study for Final Examination | 15 | 4 | 60 | |
| Contribution of Learning Outcomes to Programme Outcomes | | | * Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High |
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